What Does Research Suggest About Unexplained Infertility and Egg Quality?
August 1, 2025
August 1, 2025

Pune has built its identity around education, technology, and a pace of life that rarely slows down. For many couples here, that pace comes with a cost that shows up in unexpected places. Chronic stress, irregular sleep, long hours at a desk, and the kind of lifestyle that comes with working in IT or corporate environments are all factors that quietly affect reproductive health. When pregnancy loss happens once, it is devastating. When it happens repeatedly, and every test comes back normal, the question of why becomes harder to answer through conventional fertility investigations alone.
Finding the best miscarriage doctors in Pune means finding someone who looks at the full picture, not just the hormonal and structural one. At ICPRM, we specifically work with couples facing recurrent miscarriage where the cause has not yet been clearly identified. Our focus is reproductive immunology, and what we look for is whether the immune system is playing a role in why pregnancies are not continuing, a cause that rarely shows up on standard panels but is more common than most people realize.
Research from NICHD has documented how chronic stress disrupts reproductive hormones, affecting ovulation, implantation, and the body's ability to sustain early pregnancy. In a city like Pune, where work pressure is consistent and rarely lets up, elevated cortisol levels over time can create a hormonal environment that is quietly working against pregnancy. This is not about any single stressful event but the cumulative effect of months and years of sustained pressure.
Sleep is another factor that does not get nearly enough attention in fertility care. A systematic review on sleep disturbances and female infertility found meaningful links between poor sleep quality and worse fertility outcomes, with disrupted circadian rhythms affecting hormone regulation and increasing inflammatory markers. The NIH has also noted that chronic sleep disruption has measurable effects on immune function. For professionals in Pune working late shifts or irregular hours, this adds another layer of reproductive risk that is easy to overlook.
Sustaining a pregnancy requires more from the immune system than most people know. Because the embryo carries the father's genetic material, it is immunologically different from the mother's own cells. For the pregnancy to continue, the maternal immune system must recognize this and actively hold back from responding to the embryo as a foreign body. When that process does not work correctly, repeated pregnancy loss can follow, even when every other aspect of fertility appears completely normal.
What makes this particularly difficult to catch is that immune dysfunction is not part of standard miscarriage investigations. Natural killer cell activity, inflammatory cytokine levels, and other markers of immune dysregulation require specific testing to identify. Couples can go through complete hormonal panels, genetic testing, and uterine assessments, receive normal results across all of them, and still not have an answer. That is precisely where reproductive immunology becomes relevant.
At ICPRM, we do not begin with a generic fertility protocol. Every couple we see with a history of repeated miscarriage goes through a focused immunological evaluation first. Dr. Mugdha Raut and Dr. Mohan Raut, both Consultant Gynaecologists and Clinical Reproductive Immunologists, review each case specifically for signs of immune-related pregnancy failure. The aim is to find a cause that is specific and treatable, not to repeat investigations that have already been done.
When immune dysregulation is identified, our treatment is ImmuLIT®, a patented Lymphocyte Immunization Therapy. The procedure uses the father's prepared lymphocytes to help the maternal immune system develop the tolerance it needs to sustain the pregnancy. It is completed in a single outpatient sitting of approximately three hours. Among couples who are appropriate candidates and complete the full protocol, we see an 80% success rate. As a miscarriage treatment specialist in Pune, our focus is on getting to the right answer before recommending any treatment at all.
Here is how the process works from start to finish, designed to be manageable even for busy working couples.
Many couples who reach us have spent months, sometimes years, looking for an explanation that standard fertility care has not been able to provide. They have good reports, they have tried the recommended treatments, and they still do not have a clear answer. Our scope at ICPRM is deliberately focused on exactly that kind of case. We are located at The NewLife Hospital, Meera Multispeciality, Shankarsheth Road, Bhawani Peth, Pune, making us accessible for patients from across the city and from nearby areas.
For couples who cannot take extended time away from work, our process is designed with that in mind. The initial consultation happens online, which means you can discuss your case with us without needing to take time off. The ImmuLIT® procedure itself is a single sitting, so there are no prolonged hospital stays involved. As experienced miscarriage doctors in Pune, our entire clinical attention is on recurrent miscarriage and immune-related fertility failure, and that focus is what we bring to every case we see.
1. Can chronic stress affect pregnancy continuation?
Yes. Sustained high stress levels elevate cortisol, which can interfere with reproductive hormones and disrupt the body's ability to support early pregnancy. This is particularly relevant for professionals in high-pressure work environments where stress is consistent rather than occasional.
2. Does poor sleep impact reproductive health?
It can. Research has linked chronic sleep disruption to hormonal imbalance, increased inflammation, and poorer fertility outcomes. Irregular or insufficient sleep affects the same biological systems involved in ovulation, implantation, and immune regulation.
3. Why do miscarriages happen despite normal fertility reports?
Standard fertility panels assess hormones, uterine structure, and chromosomes. They are not designed to detect immune dysfunction. Many couples with recurrent miscarriage have entirely normal conventional results because the immune cause requires different, more specific testing to identify.
4. Can immune-related miscarriage be diagnosed through routine tests?
No. Immune causes of pregnancy loss, such as abnormal NK cell activity or cytokine dysregulation, require dedicated immune investigations. These are not part of standard miscarriage or fertility panels and need to be requested specifically.
5. What is the purpose of NK cell testing?
Natural killer cells play a role in how the immune system responds to the embryo. Elevated or dysregulated NK cell activity has been associated with recurrent miscarriage and implantation failure. Testing NK cell levels helps identify whether immune dysfunction is contributing to pregnancy loss.
6. Is ImmuLIT® used alongside IVF treatment?
ImmuLIT is an immune-focused treatment, not a replacement for IVF. In cases where immune dysregulation is identified in patients also undergoing IVF, ImmuLIT may be recommended to improve the immune environment before or during IVF cycles. The approach is determined individually based on each couple's immune evaluation.
7. Can working professionals manage the ImmuLIT® treatment without long hospital stays?
Yes. The ImmuLIT procedure is an outpatient treatment completed in a single sitting of approximately three hours. There is no hospitalization required, and the process is structured to minimise the number of visits needed, beginning with an online consultation that can be done from anywhere.

M.D.D.G.O, FCRI
Consultant Gynaecologist & Obstetrician
Clinical Reproductive Immunologist

M.D., D.G.O., F.C.R.I.(ASRI)
Consultant Gynaecologist & Obstetrician
Clinical Reproductive Immunologist